How To Please Ron/Brain
- Present about cell models
- COMPLETE PARTS LIST ON WIKI
miRNA Sensors (Theory)
- Prep experiments
- Contact Jeremy about multiple miRNA target sites chained together
miRNA Sensors (Lab Work)
- Pick colonies for MAV1212
- Miniprep MAV1212
- Keep MAV1212-Hef1a-eBFP2 (for use WITHOUT target sites)
- Gateway MAV1212-Hef1a-L7ae (for high sensor)
- Gateway MAV1212_Hef1a-eYFP (for use WITH target sites)
- Transform Hef1a, MAV1212 ( ? )
NOTES:
No MAV1212 colonies grew on the plates or in the medium
Jeremy gave us more, enough for one more transformation