Page History

Long Term Goals

miRNA Profile

•  Read literature
•  Choose profile

Building the Cell Detector Circuit

•  Build mathematical model
•  Make test bed
  •  Controlled miRNA expression
  •  Constructed miRNA sensors

Mini experiments

• •  Single miRNA detector circuit

Alzheimer's Model

•  Agree on a team wide, full Alzheimer's model
  

Today's Agenda

How To Please Ron/Brain

•  Create a clear workflow
•  Update "status" of plasmids on parts list
•  How do we string multiple low sensors together?

miRNA Sensors (Theory)

•  Determine which pairs of target sites we want
  •  Find sequences (hopefully under 200 bp)
  •  Send ultramer order to Brian

miRNA Sensors (Lab Work)

•  Tranform LRs 
  •  MAV1212-Hef1a-mKate2
  •  MAV1212-Hef1a-L7ae
  •  Hef1a on Kan
•  Pick colonies from transformations
  •  MAV1212-Hef1a-eBFP2
•  Golden Gate
  •  Gather miRNA target sites from Jeremy
  •  Make all 6 single low sensors

NOTES

MAV1212 didn't grow (ccdB resistance is over expressed)

Hef1a was plated on Amp plates...but it has Kan resistance

Verify MAV1212-Hef1a-eBFP2 via sequencing AmpR/eBFP2 region