| People to Contact | Questions | Contacted Yet? | |
|---|---|---|---|
Jacob Beal | Had first meeting | ||
Jeremy Gam | Questions | In close contact |
Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- AD blood cells from PrecisionMed? Talk to Brian?
- Meet with Kyle about designing siRNA (email sent)
- Make siRNA sequences in geneious
- Send order to Brian
- Picture of how low sensors come together
miRNA Sensors (Theory)
- Determine which pairs of target sites we want
- Find sequences (hopefully under 200 bp)
- Send ultramer order to Brian
miRNA Sensors (Lab Work)
- Golden Gate
- Gather miRNA target sites from Jeremy
- Nanodrop for concentrations ( ? )
- Make all 6 single low sensors (in conjunction with antibody group)
- Gather miRNA target sites from Jeremy
- Create new MAV vector?
- Miniprep eBFP2
- Pick colonies from mKate2 and L7ae
NOTES