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Primers

Put recently arrived primers in solution to 100uM using TE buffer (added and vortexed).

 

PCR

Ran PCRs for the following samples:

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STEP 
TEMP 
TIME 
Initial Denaturation98°C30 seconds
35 Cycles98°C
60°C
72°C
10 seconds
30 seconds
60 seconds
Final Extension72°C5 minutes
Hold4°C 

 

Electrophoresis Gel

Prepared electrophoresis gel according to protocol: Gel Extraction (includes making gels)

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