Primers
Put recently arrived primers in solution to 100uM using TE buffer (added and vortexed).
PCR
Ran PCRs for the following samples:
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STEP | TEMP | TIME |
Initial Denaturation | 98°C | 30 seconds |
35 Cycles | 98°C 60°C 72°C | 10 seconds 30 seconds 60 seconds |
Final Extension | 72°C | 5 minutes |
Hold | 4°C |
Electrophoresis Gel
Prepared electrophoresis gel according to protocol: Gel Extraction (includes making gels)
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