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People to ContactEmailQuestionsContacted Yet?

Jacob Beal

jakebeal@bbn.com

Questions

Had first meeting

Jeremy Gam

jgam@mit.edu

QuestionsIn close contact

 

Long Term Goals

miRNA Profile

  •  Read literature
  •  Choose profile

Building the Cell Detector Circuit

  •  Build mathematical model
  •  Make test bed
    •  Controlled miRNA expression
    •  Constructed miRNA sensors

Mini experiments

    •  Single miRNA detector circuit

Alzheimer's Model

  •  Agree on a team wide, full Alzheimer's model

Today's Agenda

How To Please Ron/Brain

  •  Keep updating the parts list
  •  Strengthen experiments page

miRNA Sensors (Theory)

  •  Prepare protocol for building new MAV
  •  Prepare protocol for building high sensors
  • Meet w/ Brian about double input low sensor

miRNA Sensors (Lab Work)

  •  Transform Pick low sensor Golden Gatescolonies
  •  Start building new MAV

NOTES

Meet w/ Brian tomorrow to talk about double input ultramer

Co-transfect 2x Kturn & MAV w/ target sites & eBFP2

  • mRFP cassette PCR (if primers are here)

NOTES

 PCR for building new MAV