| People to Contact | Questions | Contacted Yet? | |
|---|---|---|---|
Jacob Beal | Had first meeting | ||
Jeremy Gam | Questions | In close contact |
Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts list
miRNA Sensors (Theory)
- Meet w/ Brian about double input low sensor
- Fill out PCR protocol to be ready for tomorrow
- Create high sensor construction map (unified to look like the other groups)
- Design primers / find restriction enzymes to sequence low sensors
- Find restriction enzymes to digest original EF
miRNA Sensors (Lab Work)
- PCR mRFP out of pL1F_1_S6_S7
- Pick multiple low sensor colonies
- Re-transform low sensors
NOTES
Double input low sensor will be a gBlock
- colonies from retransformed EF and GH
- Miniprep original EF
- Restriction digest original EF
- Prepare original EF for sequencing
NOTES
Lots of blue colonies, not many white colonies (none from GH)