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Results

 

lanetubeenzyme
Lane12345678910111213141516 17 18 19 20
 tube Hyper Ladder         
enzyme               
result(P/F)               
lane123456789101112131415
GG DONRpENTRSyk-eYFP 1pENTRSyk-eYFP 2pENTRSyk-eYFP 3pENTRSyk-eYFP 4pENTR Syk tev 1pENTR Syk tev 2pENTR Syk tev 3pENTR Syk tev 4Syk+stop 1Syk+stop 2Syk+stop 3Syk+stop 4Syk+stop 5pENTR Lyn TEV 1pENTR Lyn TEV 2pENTR Lyn TEV 3pENTR Lyn TEV 4Hyper Ladder
enzyme NsiINsiINsiINsiINsiINsiINsiINsiINsiINsiINsiINsiINsiINsiIPvuIIPvuIIPvuIIPvuII 
Result(P/F)

tube

                    
       <insert gel
 Lane 2122232425262728 
result(P/F)               
lane123456789101112131415
tube               
293031323334353637383940
TubeHyper LadderGG DONRpENTR Lyn-mKate 1pENTR Lyn-mKate 2pENTR Lyn-mKate 3pENTR Lyn-mKate 4pENTR Lyn-stop 1pENTR Lyn-stop 2pENTR Lyn-stop 3pENTR Lyn-stop 4CD79A-tcs-gal4 1CD79A-tcs-gal4 2CD79A-tcs-gal4 3CD79A-tcs-gal4 4CD79A-tcs-gal4 5CD79A-stop 1CD79A-stop 2CD79A-stop 3CD79A-stop 4CD79A-stop 5
Enzyme PvuIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuIIPvuII
Result(P/F)enzyme               result(P/F)               

<Gel image here>

Plasmids

 

pENTR CD79A+STOPpENTR CD79A-TCS-Gal4VP16pENTR Syk-eYFPpENTR Syk+STOPpENTR Syk-TEVpENTR Lyn-TEVpENTR Lyn+STOPpENTR Lyn-mKateFail state (ggDONR)