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People to ContactEmailQuestionsContacted Yet?

Jacob Beal

jakebeal@bbn.com

Questions

Had first meeting

Jeremy Gam

jgam@mit.edu

QuestionsIn close contact

 

Long Term Goals

miRNA Profile

  •  Read literature
  •  Choose profile

Building the Cell Detector Circuit

  •  Build mathematical model
  •  Make test bed
    •  Controlled miRNA expression
    •  Constructed miRNA sensors

Mini experiments

    •  Single miRNA detector circuit

Alzheimer's Model

  •  Agree on a team wide, full Alzheimer's model

Today's Agenda

How To Please Ron/Brain

  •  Keep updating the parts tree

miRNA Sensors (Theory)

  •  Propose experiments to test L7ae and Tau14/21
  •  Find restriction enzymes to digest original EF

miRNA Sensors (Lab Work)

  •  PCR mRFP out of pL1F_1_S6_S7
  •  Pick colonies from retransformed EF and GH
  •  Miniprep original EF
  •  Restriction digest original EF
  •  Prepare original EF for sequencing

NOTES