LR 06/16:
LR
Transformation 06/18
Pick colonies
Miniprep, digest, sequence
Midiprep
transformation
pick colonies
miniprep
digest + gel
potential mix up in loading the gel, diges+gel repeated
digest + gel
reLR
digest + gel of LR1 with different enzymes
cultured and miniprep LR2
midi culture
midi
Gel
 | 6/23 | Notes: Inconclusive - could potentially be a failed LR with extra bands corresponding to a partial digestion. We were concerned that gel may have been loaded in an order different to what was recorded |
| 6/25
| Notes: Band pattern doesn't match either failed or successful LR. could potentially be a partial digestion with the top band at around 7kb (the size of the plasmid) and the bottom two bands being the correct digestion. |
|  | Correct. |
Sequencing results