Repeated digest
Ladder | pEXPR 1-Hef1a:LacI-2 | pEXPR 1-TRE mKate-2 | pEXPR 1-pLac mKate-2 | |
|---|---|---|---|---|
| ApaI | SalI | ApaI | ladder, h:LacI, TRE:mK, pLac:mK, syk, tevp ttevp | |
 |  |  |  |  |
| tube name | Constants Definition | pEXPR hef1A:LacI | pEXPR TRE:mKate | pEXPR pLac:mKatecd79A-3t3-gal4Vp16 | |
| tube# | 1 | 2 | 3 | ||
| gel lane | 0 | 1 | 2 | 34 | |
| DNA mass desired (ng)500 | 500 | 500 | 500 | 500 | |
| total volume(ul)20 | 20 | 20 | 20 | 20 | |
| DNA concentration (ng/ul) | 243.5 | 274.9 | 238.5 | 18 | |
| Enzyme Name | ApaI | SalI | ApaI | BglII,XbaI | |
| Buffer Name | CutSmart | 3.1 | CutSmart | 3.1 | |
| gel/lane | 0 | 1 | 2 | 34 | |
| DNA volume | 2.05 | 1.82 | 2.1027.78 | ||
| buffer volume | 2 | 2 | 2 | 2 | 2 |
| enzyme volume | 1 | 1 | 1 | 1 | 1 |
| strip tube # | 1/1 | 1/2 | 1/3 | 1/4 | |
| water volume | 17.0 | 14.9 | 15.2 | 14.9 | -10.8 |
| gel/lane | 0 | 1 | 2 | 34 | |
| loading dye4 | 4 | 4 | 4 | 4 | |
| results | fail | fail | fail | ||
| notes |