Phage display libraries are typically screened by affinity selection in ELISA plates. Currently (as of 5/23/2012) we carry capture plates for T7 from Novagen as well as polyclonal antibodies against pIII of M13 (or fd) from Novus Biologicals. The T7 capture plates are stored in the general - 4 degree fridge and the antibodies are stored in the general -20 degree fridge.
I used the following protocol to create anti-fd plates. The plates I made caught about 55 % 55% of phage when incubated with 100 uL of 1E9 TU /mL of f88/6mer library for 3 hours at 37 degrees. I suspect that tweeks could be made to this protocol to increase the capture capacity (for example, maybe not using blocking reagent).
Protocol for preparing the anti-fd plates:
1. anti-pIII is at 3.8 mg/mL (general -20 fridge). Dilute this in TBS to make 10 ug/mL solution.
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