Description:
In this experiment, we are testing whether or not beta amyloid activates the receptors, recruiting TEVp-Cofilin and leading to higher TCS cleavage and in turn, higher Gal4UAS activation. From experiment D, we determined that the level of TEVp-Cofilin expression corresponding to 0 nM dox was the level at which there was the least background TCS cleavage. We are, therefore, using TRE:TEVp-Cofilin with no dox induction in our experiment.
LilrB2:
| Expand | |||||||||||||||
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PirB:
| Expand | ||
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Dummy 200ng
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Gal4UAS:mKate200ng
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Well 12
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Results:
PirB:
| Controls | Well |
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Well 18
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1HEK293 | Well 2HEK293 hEF1a:eBFP 200ng hEF1a:PirB 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin200ng | Well 3HEK293 hEF1a:eBFP 200ng hEF1a:PirB 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin200ng | Well 4HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng Gal4UAS:mKate200ng Dummy 200ng | Well 5HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng Gal4UAS:mKate200ng Dummy 200ng 500 nM aBeta | |
 |  |  |  |  | |
| 0 nM Dox | 1 nM Dox | 10 nM Dox | 1000 nM Dox | ||
| - aBeta |  |  |  |  | |
| + aBeta |  |  |  |  |
Means:
No TEvp-Cofilin Transfected
| - aBeta | + aBeta |
|---|---|
Mean: 5827
| Mean: 6840
|
Transfected with everything
Non blue population gated out
Red histogram shown; mean = mean red
| 0 nM Dox | 1 nM Dox | 10 nM Dox | 1000 nM Dox | |
| - aBeta | Mean: 9921
| Mean: 8750
| Mean: 8707
| Mean: 8020
|
| + aBeta | Mean: 8861
| Mean: 8037
| Mean: 8108
| Mean: 7988
|
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Well 19
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Well 20
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Well 21
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Well 22
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Well 23
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