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Antibody/antigen choices - contains all of the planning info for gBlocks and antibody/antigen choices

List of secondary antibodies in Brian's fridge

Antigen binding - plans for how to detect antigen binding to mIgM (antibodies, conjugation, etc.), also contains FINAL PURCHASE DECISIONS

HIV1 gp120 (HIV envelope glycoprotein)

gp120 is a glycoprotein derived from gp160, which is cleaved into gp120 and gp41.

mAb database:  HIV Molecular Immunology 2002 (same as this)

mAb name:  0.5beta

Sequence:  IMGT

Epitope (recognition sequence):

IMGT:  RKSIRIQRGPGRAFVTIG

HIV molecular immunology database:  RGPGRAFVTIG

Peptide suppliers:

Full-length gp120 peptides are not readily available.  Check the manufacturing details to ensure that the epitope is included in the peptide.  Also check that peptide comes glycosylated.

Abcam:  10ug/$228

MyBioSource:  50ug/$365