Phage display libraries are typically screened by affinity selection in ELISA plates. Currently (as of 5/23/2012) we carry capture plates for T7 from Novagen as well as polyclonal antibodies against pIII of M13 (or fd) from Novus Biologicals. The T7 capture plates are stored in the general 4 degree fridge and the antibodies are stored in the general -20 degree fridge.
I used the following protocol to create anti-fd plates. The plates I made caught about 55% of phage when incubated with 1E9 TU of f88/6mer library for 3 hours at 37 degrees. I suspect that tweeks could be made to this protocol to increase the capture capacity.
Protocol for preparing the anti-fd plates:
1. anti-pIII is at 3.8 mg/mL (general -20 fridge). Dilute this in TBS to make 10 ug/mL solution.
2. Put 100 uL of the solution into each of the wells of a 96 well plate. parafilm the plates (to prevent evaporation) and leave at 4 degrees overnight.
4. wash plates 4 times with TBS.
5. fill with 200 uL of blocking solution (I used PBS with 1 % BSA). parafilm the plates (to prevent evaporation) and leave at 4 degrees overnight.
6. wash plates 4 times with TBS. Store dry at 4 degrees.