To transform | Plated | Mini culture | Miniprepped | Sequencing | Repeat seq | Midi culture | Frozen midipreps | Midis in sequencingseq | Completed midipreps | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CD79A-G4 |
| A15T9 | A3T15 A15T9 | A3T15 (#2) - sent 8/17 | A9T15 (sequence after) [-80] |
| |||||||||||||
CD79B-G4 |
B3T15 B9T9 | B9T15 B12T9 B15T9 |
|
| B3T15 (#1) - resent B9T9 #2 - 8/ | 1726 |
| B15T15 |
|
| B6T9 (sequence after) [-80] B6T12 (sequence after) [-80] B9T12 [-80] B12T12 [-80] B15T3 [-80] |
|
| ||||||
Syk-tTEV | S9tTEV [-80] (digest after) S12tTEV [-80] (digest after) |
| Digest these:
| ||||||||||||||||
Syk-TEV | S6TEV - sent 8/18 | S3TEV [-80] (digest after) | S9TEV [-80] (digest after) S12TEV [-80] (digest after) |
| Digest these:
| ||||||||||||||
Misc | pENTR VP16Gal4 | pENTR VP16Gal4 |