Child pages

Antibody/antigen choices - contains all of the planning info for gBlocks and antibody/antigen choices

List of secondary antibodies in Brian's fridge

Antigen binding - plans for how to detect antigen binding to mIgM (antibodies, conjugation, etc.), also contains FINAL PURCHASE DECISIONS

Chicken Lysozyme

Chicken lysozyme derives from chicken eggs.  It is used for 

Peptide suppliers:

Sigma-Aldrich:  1g/$49.50

Merck-Millipore:  1g/$39

HIV1 gp120 (HIV envelope glycoprotein)

gp120 is a glycoprotein derived from gp160, which is cleaved into gp120 and gp41.

mAb database:  HIV Molecular Immunology 2002 (same as this)

mAb name:  0.5beta

Sequence:  IMGT

Epitope (recognition sequence):

IMGT:  RKSIRIQRGPGRAFVTIG

HIV molecular immunology database:  RGPGRAFVTIG

Peptide suppliers:

Full-length gp120 peptides are not readily available.  Check the manufacturing details to ensure that the epitope is included in the peptide.  Also check that peptide comes glycosylated.

Abcam:  10ug/$228

MyBioSource:  50ug/$365