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06/16:

LR

06/18

transformation

06/19

pick colonies

06/22

miniprep

06/23

digest + gel

potential mix up in loading the gel, diges+gel repeated

06/25

digest + gel

reLR

06/29

digest + gel of LR1 with different enzymes

cultured and miniprep LR2

Gel

Image Added

6/23

Image Added

Notes:

Correct band pattern with the exception of the lowest band at around 1.2 kb. This band pattern looks very similar to the expected band pattern of another sample that was loaded on the same gel - which is consistent with the samples being loaded in the wrong order.

Image Added

6/25Image Added

 

Notes:

idk...

 

Sequencing results