...
Try every combination of Gal4VP16 once with beta amyloid and once without.
Put Syk should be onon dox inducible promoter (with mKate or something to measure Syk) and make a dox ladder for each test group to determine activity level.
| Standardized? Standardized information | ||||||
|---|---|---|---|---|---|---|
| Plasmids used | Protocols | Supplies | Control | Predicted results | Results | What this means |
CD79A CD79A-TCS-Gal4CP16 CD79B CD79B-TCS-Gal4CP16 Lyn dox inducible promoter Syk-15-TEVp dito mKate | ||||||
| Beta Amyloid? | Gal4VP16 on CD79A | Gal4V16 on CD79B |
|---|---|---|
0 | 0 | 0 |
| 0 | 0 | 1 |
| 0 | 1 | 0 |
| 0 | 1 | 1 |
| 1 | 0 | 0 |
| 1 | 1 | 0 |
| 1 | 0 | 1 |
| 1 | 1 | 1 |