PCR of TCS-Gal4VP16
Gel shows successful PCR of TCS-Gal4VP16 from pEXPR hEF1a-FGFR-mKate-TCS(L)-G4.
Forward primer: iGEM KRB 001 F
Reverse primer: iGEM KRB 002 R
Annealing Temperature: 63C?
Run using samples after PCR purification.
PCR of CD79A-TCS
Gel shows successful PCR of CD79A-TCS from pENTR CD79A (pDONR223).
Forward primer: iGEM KRB 003 F
...
PCR purified 23 June 2014.
Restriction Digest Verification of pENTR
1 July 2014:
See gel here
Correct plasmids | Failed plasmids |
|---|---|
CD79A-TCS-G4 (1) CD79A-TCS-G4 (2) CD79A-TCS-G4 (5) | CD79A-TCS-G4 (3) CD79A-TCS-G4 (4) |