PCR of TCS-Gal4VP16

Gel shows successful PCR of TCS-Gal4VP16 from pEXPR hEF1a-FGFR-mKate-TCS(L)-G4.

Forward primer:  iGEM KRB 001 F

Reverse primer:  iGEM KRB 002 R

Annealing Temperature:  63C?

Run using samples after PCR purification.

PCR of CD79A-TCS

Gel shows successful PCR of CD79A-TCS from pENTR CD79A (pDONR223).

Forward primer:  iGEM KRB 003 F

Reverse primer:  iGEM KRB 005 R

Annealing temperature:  60C

Run using samples before PCR purification.

PCR purified 23 June 2014.

Restriction Digest Verification of pENTR

1 July 2014:

See gel here