| To transform | Plated | Mini culture | Miniprepped | Sequencing | Repeat seq | Midi culture | Frozen midipreps | Midis in seq | Completed midipreps | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CD79A-G4 | A15T9 |
|
| A3T15 | A9T15 - repeat seq 8/22 |
| |||||||||||
| CD79B-G4 |
| B3T15 B9T9 B9T15 B12T9 B15T9 |
B15T15 | B9T9 #2 - 8/26 |
| B15T15 |
|
|
| B6T12 - resent 8/22 |
| ||||||
| Syk-tTEV |
| Digest these:
| |||||||||||||||
| Syk-TEV | S9TEV | S6TEV S9TEV |
| Digest these:
| |||||||||||||
| Misc | pENTR VP16Gal4 |
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