Primers
Put recently arrived primers in solution to 100uM using TE buffer (added and vortexed).
PCR
Ran PCRs for the following samples:
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| STEP | TEMP | TIME |
| Initial Denaturation | 98°C | 30 seconds |
| 35 Cycles | 98°C 60°C 72°C | 10 seconds 30 seconds 60 seconds |
| Final Extension | 72°C | 5 minutes |
| Hold | 4°C |
Electrophoresis Gel
Prepared electrophoresis gel according to protocol: Gel Extraction (includes making gels)
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Lane 2: CD79A + stop codon (724bp)
Lane 3: CD79A + TCS (718bp)
Lane 4: linker + mKate (768bp)
When loading wells with PCR products, used Parafilm to mix 1uL of 6X Orange dye with 5uL of sample and loaded 5uL of this mixture in a lane. Ran gel at 120V for 30 minutes.
