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Instructions for newbies:
1.5 uL of 10 fM fmol/uL Dest
1.5 uL of 5 fM fmol/uL promoter
1.5 uL of 5 fM fmol/uL gene
1.5 uL of H2O
1.5 uL of LR clonase
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Combine into 1 aliquot:
- 1uL of 5 fmol/uL of pENTR_L4_Promoter_R1
- 1uL of 5 fmol/uL pENTR_L1_Gene_L2
- 1uL of 10 fmol/uL pDEST_R4_R2
- 3uL Total
WARNING: KEEP ALIQUOTED LR CLONASE MIX AT -80 AT ALL TIMES!
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