Description:
In this experiment, we are testing whether or not beta amyloid activates the receptors, recruiting TEVp-Cofilin and leading to higher TCS cleavage and in turn, higher Gal4UAS activation. From experiment D, we determined that the level of TEVp-Cofilin expression corresponding to 0 nM dox was the level at which there was the least background TCS cleavage. We are, therefore, using TRE:TEVp-Cofilin with no dox induction in our experiment.
LilrB2:
...
| Expand | ||
|---|---|---|
|
...
|
...
|
...
|
...
|
...
|
...
|
...
|
...
|
...
|
...
7.8125 nM abeta
Well 6
|
|
...
|
...
|
PirB:
| Expand | ||||
|---|---|---|---|---|
| ||||
|
...
|
...
|
...
|
...
|
...
|
...
|
...
|
...
|
...
|
...
|
...
|
...
|
...
|
...
|
...
|
...
|
...
|
...
|
...
|
...
|
...
Well 13
...
Well 14
...
Well 15
...
Well 16
...
Well 17
...
Well 18
...
Well 19
...
Well 20
...
Well 21
...
Well 22
...
Well 23
...
|
Results:
PirB:
| Controls | Well 1HEK293 | Well 2HEK293 hEF1a:eBFP 200ng hEF1a:PirB 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin200ng | Well 3HEK293 hEF1a:eBFP 200ng hEF1a:PirB 200ng hEF1a:rtTA 200ng TRE:TEVp-Cofilin200ng | Well 4HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng Gal4UAS:mKate200ng Dummy 200ng | Well 5HEK293 hEF1a:eBFP 200ng hEF1a:PirB-TCS-Gal4VP16 200ng hEF1a:rtTA 200ng Gal4UAS:mKate200ng Dummy 200ng 500 nM aBeta |
 |  |  |  |  | |
| 0 nM Dox | 1 nM Dox | 10 nM Dox | 1000 nM Dox | ||
| - aBeta |  |  |  |  | |
| + aBeta |  |  |  |  |
Means:
No TEvp-Cofilin Transfected
| - aBeta | + aBeta |
|---|---|
Mean: 5827
| Mean: 6840
|
Transfected with everything
Non blue population gated out
Red histogram shown; mean = mean red
| 0 nM Dox | 1 nM Dox | 10 nM Dox | 1000 nM Dox | |
| - aBeta | Mean: 9921
| Mean: 8750
| Mean: 8707
| Mean: 8020
|
| + aBeta | Mean: 8861
| Mean: 8037
| Mean: 8108
| Mean: 7988
|
