| People to Contact | Questions | Contacted Yet? | |
|---|---|---|---|
Jacob Beal | Had first meeting | ||
Jeremy Gam | Questions | In close contact |
Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Find all the plasmids (and maps) we need for experiments
- Update experiments page
- Talk to Gin? about miRNA target sites and TAL14
- Talk to Lyla and Alexa about Transfection schedule
miRNA Sensors (Lab Work)
- Transformations
- Low Sensor Golden Gates
- pEXPR: Hef1a-eBFP2 (186 ng/uL)
- pEXPR: Hef1a-L7ae (96 ng/uL)
- Pick red colonies and grow in medium!
- Find concentrations of eBFP2 and L7ae
- Grow up pEXPR Hef1A:EBFP2
- Find UAS eYFP (talk to B Cell Receptor Group)
- TAL14, Gal4VP16 (Find a way to talk to G/Jin)
- Find Hef1A 2xKturn eYFP (Nelson)
NOTES
Nelson doesn't know where it is, but he needs it as well, so he's on the hunt