Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Troubleshoot
- What's the deal with the GAL4VP16?!
miRNA Sensors (Lab Work)
- Midi prep??
- pEXPR: MAV1212-hEF1a-TAL14
- Pick colonies
- pEXPR: MAV1212-TRE-L7ae (LR from last night)
- Proteinase K
- MAV1212-hEF1a-TAL21
- Transform
- MAV1212-hEF1a-TAL21
- Miniprep
- Low Sensor miR-144
- Low Sensor miR-181c
- High Sensor miR-30d
- High Sensor miR-125b
- High Sensor miR-146a
- High Sensor let-7f
- Send for sequencing
- Low Sensor miR-144
- Low Sensor miR-181c
- High Sensor miR-30d
- High Sensor miR-125b
- High Sensor miR-146a
- High Sensor let-7f
- Analyze sequencing results
- Low Sensor miR-144
- Low Sensor miR-181c
- High Sensor miR-30d
- High Sensor miR-125b
- High Sensor miR-146a
- High Sensor let-7f
- Transfect
- L7ae experiment (Take 3)
NOTES
pEXPR: MAV1212-hEF1a-GAL4VP16 and pEXPR: MAV1212-TRE-GAL4VP16 only had blue colonies, which doesn't make sense because they were both verified...
Brian is in the middle of something on his bench, so we won't midi today