Long Term Goals
Building the Cell Detector Circuit
- Choose profile
- Verify sensors
- Create mathematical model
Experiments
- L7ae repression
- TAL14 repression
- Single input sensors
- Multiple input sensors
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Get TAL21 promoter from Brian?
- Plan experiments
- DOX induction for L7ae repression (look here)
- Tuning TAL14 repression
- Learn about gating and FACS analysis
miRNA Sensors (Lab Work)
- Midi prep
- pEXPR: MAV1212-TRE-L7ae
- Mini prep
- MAV1212-hEF1a-TAL21
- MAV1212-hEF1a-TAL14
- Low Sensor miR-144-181c
- Sequence verify
- Low Sensor miR-144-181c
- Digest and run gel
- MAV1212-hEF1a-TAL21
- MAV1212-hEF1a-TAL14
- Transform
- All the TAL14 low sensors
NOTES