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hEF1a:CD79B-pTet:mRFP-Gal4VP16

PCR: when we redesigned primers to fix dimerization issues, we accidentally introduced a missense mutation in the third codon from the end of CD79B. We decided to use SDM to fix the mutation instead of rePCRing

06/19

  • SDM

06/23

  • Gel verification of SDM --> no bands: possible that there wasn't enough amplification. will run SDM again and transform product directly 

hEF1a:CD79B-XnTCSXc-Gal4VP16

3TCS3 9TCS3 15TCS3
     
 6TCS69TCS612TCS6 
     
3TCS96TCS99TCS912TCS915TCS9
     
 6TCS129TCS1212TCS12 
     
3TCS15 9TCS15 15TCS15
     
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