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Take DNA, determine concentration and length (using NanoDrop and Geneious/Gel)

Determine ng of DNA needed to create 40 fmol solutions.

ng = amount in fmol x length of plasmid in bp x 0.00066 (ng / fmol )
(Check formula using Google)

100ng DNA
5ul each gBlock @ 40fmol each
100ng Donor
2ul 10X T4 Ligase Buffer
2ul 10X BSA
1ul BsaI-HF
1ul T4 Ligase HC
fill to 20uL with SDIH20
-------------
20ul total

protocol EBGG
37 5min

50X:
37 2.5min
4C 0.5min
16C 5.5min
37C 10 min
80C 20 min

4C hold

takes 8+ hours
(check protocol by looking up the paper or other online GG protocols)

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