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In this experiment, we will determine the affinity of beta-amyloid for the variable regions of our mIgM in the B-cell receptor.
We plan to determine whether beta-amyloid binds to our BCR. We would also like to establish a Kd value if possible.
Different concentrations of beta-amyloid...
(for each concentration also use a nontransfected control to measure background binding)
http://www.renyi.hu/~stipsicz/skin/Flow_cytometry_book/Ch_17.pdf p.16-19 - protocol for yeast using c-myc as transfection marker