- Safety - get trained
- PRIORITY: decide which system
- 1st system: Hydrogen production from cellulose by co-culture of Clostridium thermocellum JN4 and Thermoanaerobacterium thermosaccharolyticum GD17
- 2nd system: Phototrophic hydrogen production from glucose by pure and co-cultures of Clostridium butyricum and Rhodobacter sphaeroides
- Read the papers on each system
- Make sure the bacteria are transformable
- Clostridium thermocellum JN4: https://microbewiki.kenyon.edu/index.php/Clostridium_thermocellum
- Thermoanaerobacterium thermosaccharolyticum GD17: PubMed
- Clostridium butyricum: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4204974/
- Rhodobacter sphaeroides: https://microbewiki.kenyon.edu/index.php/Rhodobacter_sphaeroides
- Talk to an expert; evaluate pros and cons of each system
- Next meeting moved from Thurs (4/9) to Sunday (4/12) at 3 pm because of 8.02 exam
- Project Structure
- Metabolism - measuring rate of glucose and lactate production so that we can model
- C. Therm:
- rate lactate production
- lactate production vs cellulose concentration
- T. Therm: H2 production vs lactate concentration
- instantaneous
- batch
- continuous culture
- C. Therm:
- Signaling - AutoInducer-2
- Make sure we can send our parts back to iGEM headquarters at end of the summer
- plasmids have to be compatible
- Senders send:
- chromatography
- Receivers receive:
- AI-2 -> GFP
- [GFP] vs [AI-2]
- Make sure we can send our parts back to iGEM headquarters at end of the summer
- Natural sensors
- Actuators: slow down growth of 1st bacterium
- cell cycle checkpoint
- mRNA endonuclease (relE)
- antibiotic synthesis?
- Modeling
- Fuel cell - not right now...
- Metabolism - measuring rate of glucose and lactate production so that we can model
- What don't we know? What's the next step?
- Prove that our system is robust to perturbation
- Temperature
- Feed stock
- density effects
- pH/anaerobic byproducts
- continuous culture - how long can it keep producing hydrogen without changing the feed stock?
- atmospheric pressure
- Industrial concerns?
- concretely specify the real world problem
- feedstock (eg running car off of old corn husk)
- economics
- scalability (our cocultures could enable better biohydrogen production at an industrial scale)
- 3rd world country
- uses: energy (fuel cells), fertilizer
- Most hydrogen is produced from fossil fuels; biohydrogen production accounts for very little
- concretely specify the real world problem
- Prove that our system is robust to perturbation
- TEAMS FOR NEXT WEEK:
- Which system? Marjorie, Ryan, Jesse, Alyssa, Nick, (Brian)
- do more research on each system: bacteria for each, required growth conditions
- Use Case: Ayesha, Wendi, Josh, Molly, Nathan, (Kyle)
- answer the question: why are we doing this?
- quotes from important people
- Which system? Marjorie, Ryan, Jesse, Alyssa, Nick, (Brian)
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