1. Make sure that jacket steam pressure is not at 0. If at 0, press the jacket steam reset button (red, in the middle).
  2. Make sure that autoclave is turned off. On/Off button is the red square in the center. 
  3. Open door. Outer lock tightens/loosens door. Inner lock opens/closes door. 
  4. Put samples inside. Make sure all caps are loose and autoclave tape is on. 
  5. Close door and tighten. Make sure to not over tighten or the handle will break. 
  6. Set the time. 
  7. Make sure the cycle is set to "Liquid Cycle".
  8. Press the "On" button. 
  9. Make sure that the temperature and pressure are rising. 
  10. After the autoclave is done, make sure that temperature is below 100C. 
  11. Turn off the autoclave. 
  12. Open the door. 
  13. Take out your sample. 
  14. Reset the jacket steam. 

 

 

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