You are viewing an old version of this page. View the current version.

Compare with Current View Page History

« Previous Version 5 Next »

Filter sets from Chroma http://www.chroma.com

5X Fluorescence Filter Cubes: (EX: Excitation, DM: Dichromatic Mirror, BA: barrier filter emission)

1. UV -> EX:350/50nm (i.e. 325-375nm), DM:400nm (longpass, LP),  BA: 420nm (longpass, LP) Dyes:DAPI, Hoechst, AMCA [11000v3-UV] 

2. FITC -> EX:HQ480/40nm (HQ stands for high quality, i.e. high S/N ratio), DM:505nm, BA: 610nm Dyes:FITC, RSGFP, Bodipy, Fluo 3, Dio, GFP, Alexa488 [41001] 

3. BLUE-PROCL-> EX: D470/40nm, DM:495nm (longpass, LP), BA: 515nm (longpass, LP) Dyes:YFP [11001v2-BLUE] 

4. GREEN-PROCL-> EX:546/10nm, DM:565nm, BA:590nm(longpass, LP) Dyes:GFP [11002v2-GREEN] 

5. HPTS-> EX:452/40nm, DM:460nm (longpass, LP), BA: D540/50 Dyes:CFP 

6. Currently Empty

c.f. To choose right filter combinations for fluorescent proteins -> http://www.microscopyu.com/tutorials/java/fluorescence/fpfilterfinder/index.html

Fluorescence Illuminator : X-cite 120 from Exfo

Objectives (Magnification / N.A.): 2X/0.08, 4x/0.13, 10x/0.30, 20x/0.45, 40x/0.60, 60x, 100x/1.40

CCD Camera :PCO 1600

  • No labels