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miRNA Sensing
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miRNA Proposed Experiments
Created by
Unknown User (gary_9@mit.edu)
, last modified on
Jul 24, 2014 09:14
TRANSFECTION PLANS
L7ae Repression
Name
Location
Concentration
MAV1212-hEF1a-eBFP2
TC Room
Midi prepped
pEXPR:hEF1a-L7ae
TC Room
Midi prepped
pEXPR: CAG-2xKturn-eGFP (wt)
TC Room
Midi prepped
pEXPR: CAG-exKturn-eGFP (mut)
TC Room
Midi prepped
EXPERIMENTS
hEF1a eBFP2 & hEF1a 2x Kturn eYFP (positive control)
Should be yellow and blue
hEF1a eBFP2 & hEF1a 2x Kturn eYFP & hEF1a-L7ae (negative control)
Should be blue (minimal yellow)
TAL14 Repression
Name
Location
Concentration
pEXPR: hEF1a-eBFP2
TC Room
Midi prepped
pEXPR: hEF1a-Gal4VP16
Our -20
Verified, colony being picked for midi prep
pEXPR: hEF1a-TAL14
Our -20
Being midi prepped
pEXPR: TAL14-mKate
TC Room
Midi prepped
EXPERIMENTS:
hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP
negative control (minimal yellow)
hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP
& hEF1a-Gal4VP16
positive control (hopefully linear BFP/YFP)
hEF1a-eBFP2
& 5xUAS/4xTAL14bs-eYFP
& hEF1a-Gal4VP16 & hEF1a-TAL14
Should be blue (minimal yellow)
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{"serverDuration": 1557, "requestCorrelationId": "d077a3e1c495f44e"}