PCR of Gmab Heavy

Gel shows successful PCR of Gmab Heavy from gBlock mIgM Gmab Heavy.

Forward primer:  gBlock F

Reverse primer:  gBlock R

Annealing temperature:  ?

Run using samples after PCR purification.  Extra bands indicated multiple PCR products, used gel extraction to purify out the appropriate band.

Restriction Digest Verification of pENTR

7 July 2014:

See gel here