For PCR Primers:

For Sequencing Primers:

General tips for sequencing:

Reads are only clear about 30 bp after the primer to 600 bp after the primer.

The easiest way to pick primers is to start with one in a 50 bp region in front of the gene and then measure out 500 bp from that primer then place the next one in the following 100 bp region then measure out 500 bp and place one in the next 100 bp region and so on.

If you are planning on doing additional cloning with this part try to make some of the primers usable for future constructs

For Both: