Melting Temperature
Link to Tm Calculator: https://www.neb.com/tools-and-resources/interactive-tools/tm-calculator
Product Group: Phusion
Product: Phusion High-Fidelity PCR Master Mix (HF Buffer)
Primer Conc. (nM): 500
For the primer sequences, input only the binding region and not the entire primer.
For PCR protocol: https://www.neb.com/protocols/2012/09/06/protocol-phusion-high-fidelity-pcr-master-mix-with-hf-buffer-m0531
Hairpins and Dimers
IDT's Oligoanalyzer: http://www.idtdna.com/calc/analyzer
How to interpret the delta G values you get: http://www.premierbiosoft.com/tech_notes/PCR_Primer_Design.html