BCR 2.0 

Experimental Progress:

Cloning Progress
Parts Tree
Cloning Strategy
Experiments
Linker Optimization
Antibody Choices 

 

Useful Links: 

Protocols
Immediate To Do's:

BCR 2.0 To Do

^^^Email: bcrpeeps@mit.edu

^^^Pass: grapefruit

TaskClaimed byDateOther NotesDone?
design CD79B SDM primersLA CRJun 16  
  • order SDM primers
CRJun 16  
  • SDM + transform
 Jun 18  
  • pick colonies
 Jun 19  
  • miniprep
 Jun 20  
  • digest
 Jun 20  
  • sequencing
 Jun 19  
LR LacI BCR Jun 16  
  • kill LR and transform
 Jun 17  
  • pick colonies
 Jun 18  
  • miniprep
 Jun 19  
  • digest
 Jun 19  
  • sequencing
 Jun 19  
TaskClaimed byDate CompletedOther Notes
Variable region cloning planning   
    
Higher Level To Do's:
  • Decide on other antigen-variable regions we want to test  
  • Other experimental planning (on experimental page)
  • How do we do site directed mutagenesis?
  • Literature research: 
    • kozak sequences for heavy and light chains and CD79s
    • truncated TEVp
    • other antigens to detect using BCR (we were thinking of small molecule detection)
    • inducible promoters (we were considering LacI - an inducible repressor - but we want to look into other options first) 
  • Cloning planning 
    • hEF1a:Syk-pTet:mRFP-TEVp
    • variable region