| Construct | F Tm | R Tm | Rxn T |
|---|---|---|---|
| CD79A | 61 | 60 | 65 |
| CD79B | 66 | 67 | 71 |
| Gal4VP16 | 66 | 62 | 65 |
| pTEt:mRFP | 51 | 58 | 61 |
| Construct | Expected Band Size |
|---|---|
| CD79A | 750 bp |
| CD79B | 750 bp |
| Gal4VP16 | 750 bp |
| pTet:mRFP | 800 bp |
| Ladder | CD79A | CD79B | Gal4VP16 | pTet:mRFP |
 |  |
Notes:
Gal4VP16 and pTet:mRFP constructs have the correct band size --> ready for AarI golden gate
CD79A and CD79B PCRs still seem to have primer dimers. CD79A/B primers have to be redesigned.