Repeated digest
Ladder | pEXPR 1-Hef1a:LacI-2 | pEXPR 1-TRE mKate-2 | pEXPR 1-pLac mKate-2 | ||
---|---|---|---|---|---|
ApaI | SalI | ApaI | BglII, XbaI | BglII, MluI | |
9 | 10 | 11 | 12 | ||
tube name | Constants Definition | pEXPR hef1A:LacI | pEXPR TRE:mKate | pEXPR pLac:mKate |
tube# | 1 | 2 | 3 | |
gel lane | 0 | 1 | 2 | 3 |
DNA mass desired (ng) | 500 | 500 | 500 | 500 |
total volume(ul) | 20 | 20 | 20 | 20 |
DNA concentration (ng/ul) | 243.5 | 274.9 | 238.5 | |
Enzyme Name | ApaI | SalI | ApaI | |
Buffer Name | CutSmart | 3.1 | CutSmart | |
gel/lane | 0 | 1 | 2 | 3 |
DNA volume | 2.05 | 1.82 | 2.10 | |
buffer volume | 2 | 2 | 2 | 2 |
enzyme volume | 1 | 1 | 1 | 1 |
strip tube # | 1/1 | 1/2 | 1/3 | |
water volume | 17.0 | 14.9 | 15.2 | 14.9 |
gel/lane | 0 | 1 | 2 | 3 |
loading dye | 4 | 4 | 4 | 4 |
results | ||||
notes |