How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Troubleshoot
- Why didn't the oligos nanodrop??
- Meet w/ Kyle about FlowJo
- Look for the siRNA
miRNA Sensors (Lab Work)
- Golden Gate
- Low Sensor miR-144-miR-181c
- Pick colonies
- High Sensor miR-30d
- High Sensor miR-146a
- High Sensor let-7f
- High Sensor miR-125b
- Low Sensor miR-144
- Low Sensor miR-181c
- Miniprep
- pEXPR: MAV1212-TRE-L7ae (LR)
- pEXPR: MAV1212-hEF1a-GAL4VP16 (LR)
- pEXPR: MAV1212-TRE-GAL4VP16 (LR)
- Restriction Digest & run gel
- pEXPR: MAV1212-TRE-L7ae (LR)
- pEXPR: MAV1212-hEF1a-GAL4VP16 (LR)
- pEXPR: MAV1212-TRE-GAL4VP16 (LR)
- Midiprep?
- pEXPR: MAV1212-TRE-L7ae (LR)
- pEXPR: MAV1212-hEF1a-GAL4VP16 (LR)
- pEXPR: MAV1212-TRE-GAL4VP16 (LR)
- Gateway
- pEXPR: MAV1212-hEF1a-TAL14
- Transform
- pENTR: TAL14
- pENTR: TAL21
- pENTR: Something for yeast for Brian
NOTES
The gel didn't work very well, so we're re-doing it tomorrow
CAG/CMV 2x Kturn Wild Type
CAG/CMV 2x Kturn Mutant
