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People to ContactEmailQuestionsContacted Yet?

Jacob Beal

jakebeal@bbn.com

Questions

Had first meeting

Jeremy Gam

jgam@mit.edu

QuestionsIn close contact
Jin Huh

jhuh@mit.edu

QuestionsHad first meeting

 

Long Term Goals

miRNA Profile

  • Read literature
  • Choose profile

Building the Cell Detector Circuit

  • Build mathematical model
  • Make test bed
    • Controlled miRNA expression
    • Constructed miRNA sensors

Mini experiments

    • Single miRNA detector circuit

Alzheimer's Model

  • Agree on a team wide, full Alzheimer's model

Today's Agenda

How To Please Ron/Brain

miRNA Sensors (Theory)

miRNA Sensors (Lab Work)

  • Midi prep
    • pEXPR: MAV1212-hEF1a-TAL14
  • Transform
    • pEXPR: MAV1212-TRE-L7ae (LR from last night)
  • Proteinase K
    • MAV1212-hEF1a-TAL14
  • Restriction Digest and run gel
    • MAV1212-hEF1a-TAL14
  • LR
    • MAV1212-hEF1a-TAL21
  • Mini prep
    • Low Sensor miR-144
    • Low Sensor miR-181c
    • High Sensor miR-30d
    • High Sensor miR-125b
    • High Sensor miR-146a
    • High Sensor let-7f
  • Send for sequencing
    • Low Sensor miR-144
    • Low Sensor miR-181c
    • High Sensor miR-30d
    • High Sensor miR-125b
    • High Sensor miR-146a
    • High Sensor let-7f
  • Pick colonies
    • Low Sensor miR-144
    • Low Sensor miR-181c
    • High Sensor miR-30d
    • High Sensor miR-125b
    • High Sensor miR-146a
    • High Sensor let-7f
    • pEXPR: MAV1212-hEF1a-GAL4VP16
    • pEXPR: MAV1212-TRE-GAL4VP16
  • Seed plates
    • L7ae experiment (Take 3)

NOTES

 

 

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