• Created by Unknown User (lylaatta@mit.edu), last modified by Unknown User (shinjini@mit.edu) on Jul 24, 2014 08:25
  • Miniprep pEXPR_hEF1a: LilrB2/PirB
  • Make Cell stocks
  • Make midiprep liquid culture innoculation
7/23
  • Pick a gazillion eYFP-cofilin golden gate colonies

7/23

Miniprep pEXPR_hEF1a: LilrB2/PirB:

A couple came out to have around 700ng/ul. Others were around 400ng/ul

Midiprep Culture:

Picked 2 out of 5 pEXPR_hEF1a: PirB's to inoculate midiprep cultures, but kept the miniprep cultures of the other 3 just in case.
Also inoculated pEXPR_hEF1a: LilrB2.
Midiprep party tomorrow!

eYFP-Cofilin:
Plate had lots of white colonies and a few blue ones. Not expected. Expecting a lot of backbone re-ligation. Picked 10. Will miniprep tomorrow. (Also realized I've been labelling the eYFP and Cofilin in the wrong order...)

 

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