Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Obtain Plasmids
- pEXPR: hEF1a-GAL4VP16
- pENTR: TRE (promoter)
- pEXPR: TAL14-mKate
- Troubleshoot
- So where are the siRNA though...
- Why didn't the GH golden gate work on try #3?
- Fill out the Genewiz Order Form to sequence Low Sensor miR-144
miRNA Sensors (Lab Work)
- Pick colonies
- pEXPR: TAL14-mKate
- LR
- pEXPR: MAV1212-TRE-L7ae
- Tranfection
- L7ae experiment
NOTES