Did midipreps. Very strange distribution of concentrations:
LilrB2 1: ~1200
LilrB2 2: ~700
PirB 7: ~4000
PirB8: ~300
Digesting midipreps just in case.
LilrB2: XbaI, ApaI
PirB: ApaI
LilrB2's are weird. Running undigested controls.
LirB2 DNA is contaminated!
Gel Extraction?
Suspecting we (I) messed up the glycerpl stock... Will gel extract tomorrow, and also transform with new DNA I KNOW are verified, and throw away old stocks.