Did midipreps. Very strange distribution of concentrations:
LilrB2 1: ~1200
LilrB2 2: ~700
PirB 7: ~4000
PirB8: ~300
Digesting midipreps just in case.
LilrB2: XbaI, ApaI
PirB: ApaI
LilrB2's are weird. Running undigested controls.
LirB2 DNA is contaminated!
Correct band pattern is most definitely there. Which means all parts: hEF1a promoter, LilrB2 gene and backbone are there.
Gel Extraction?
Will gel extract tomorrow, transform with that and also transform with new DNA I KNOW are verified(, make cell stocks of them, verify and throw away old stocks afterwards).