Long Term Goals
Building the Cell Detector Circuit
- Choose profile
- Verify sensors
- Create mathematical model
Experiments
- L7ae repression
- TAL14 repression
- TAL21 repression
- Single input sensors
- Multiple input sensors
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- What about the double input low sensor?
miRNA Sensors (Lab Work)
- Midi prep
- pEXPR: TAL21-mKate
- pEXPR: hEF1a-TAL21
- Run gel
- MAV1212-hEF1a-TAL21
- MAV1212-hEF1a-TAL14
- Golden Gate (if digest works)
- All the TAL21 high sensors
- Pick colonies
- miR-30d (TAL14)
- miR-125b (TAL14)
- let-7f (TAL14)
- DOX Induction
- L7ae tuning experiment
- Transfect
- TAL14 tuning experiment
NOTES