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To Do:

Get LRs to work

  • Transform all LRs (hEF1a:PT, hEF1a:LT, hEF1a:TC, TREt:TC)
  • Pick hEF1a:YC colonies
  • Miniprep TREt:YC if it worked. Otherwise, wait for Brian. Worked.
  • Test LilrB2TCSG4 digest in random places. Is the main LilrB2 stock missing a chunk? Preposterous.
  • PCR C term YFP?

Digest LilrB2TCSG4 with NcoI and PstI seperately.
Digest TREt:YC with ApaI

 

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