| People to Contact | Questions | Contacted Yet? | |
|---|---|---|---|
Jacob Beal | Meeting before NEGEM | ||
Jeremy Gam | Questions | In close contact |
Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
Ask Jeremy
- Why genome integration with bxb1 and puro?
- Will this work with transient transfection?
Presentations
- Double check what NEGEM looks like
miRNA Sensors (Theory)
- Learn about Jacob Beal's work and how it applies to us
- Make diagram to connect circuit diagram to plasmid construction map
miRNA Sensors (Lab Work)
- Check MAV1212 and MAV1212-Hef1a-EBFP2 plates
- Pick as many colonies as possible to grow overnight
- Miniprep them
- Sequence ccdB region
- Transform MAV1212-Hef1a-mKate2 (if ready)
- Gateway MAV1212-Hef1a-L7ae
- Find Hef1a
- Find L7ae
Cell Models
- Finish table of concentrations in each cell line
- Finalize siRNA transfection technique
Social Media
- Check if Alexa handled public wiki
- Add more to facebook page
- Keep Twitter live