miRNA To Do List

Long Term Goals

miRNA Profile

• Read literature
• Choose profile

Building the Cell Detector Circuit

• Build mathematical model
• Make test bed
  • Controlled miRNA expression
  • Constructed miRNA sensors

Mini experiments

• • Single miRNA detector circuit

Alzheimer's Model

• Agree on a team wide, full Alzheimer's model
  

Today's Agenda

How To Please Ron/Brain

• Keep updating the parts tree (include experiments)
• Meet w/ Kyle
  • FlowJo
  • Bright Field Images

miRNA Sensors (Theory)

• Troubleshoot

miRNA Sensors (Lab Work)

• Midi prep
  • pEXPR: MAV1212-hEF1a-TAL14
• Transform
  
  • pEXPR: MAV1212-TRE-L7ae
• Mini prep
  
  • Low Sensor miR-144
  • Low Sensor miR-181c
  • High Sensor miR-30d
  • High Sensor miR-125b
  • High Sensor miR-146a
  • High Sensor let-7f
• Send for sequencing
  • Low Sensor miR-144
  • Low Sensor miR-181c
  • High Sensor miR-30d
  • High Sensor miR-125b
  • High Sensor miR-146a
  • High Sensor let-7f
• Pick colonies
  
  • Low Sensor miR-144
  • Low Sensor miR-181c
  • High Sensor miR-30d
  • High Sensor miR-125b
  • High Sensor miR-146a
  • High Sensor let-7f
  • pEXPR: MAV1212-hEF1a-GAL4VP16
  • pEXPR: MAV1212-TRE-GAL4VP16
• Transfect L7ae experiment (Take 3)
• Re-dilute double input low sensor target sites??
• Golden Gate
  • Low Sensor miR-144-181c
    
    

NOTES